利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN104059994A 一种基于血清miRNA的试剂盒及其使用方法和应用 失效 - 权利终止

一种基于血清miRNA的试剂盒及其使用方法和应用
摘要：
本发明属于生物技术领域，具体涉及一种基于血清miRNA的试剂盒及其使用方法和在发热伴血小板减少综合征病毒早期感染检测中的应用。本发明涉及的miRNA分子包括hsa-miR-188-3p、hsa-miR-517a-3p、hsa-miR-518f-5p和hsa-miR-511-3p。通过Taqman低密度芯片和qRT-PCR方法，鉴别出上述4中miRNA在发热伴血小板减少综合征病毒发病早期表达特异性地显著升高。用这4种血清miRNA作为分子靶标，建立了一种快速、敏感、特异的发热伴血小板减少综合征病毒急性感染的检测方法。本发明涉及hsa-miR-188-3p、hsa-miR-517a-3p、hsa-miR-518f-5p和hsa-miR-511-3p在发热伴血小板减少综合征病毒感染早期诊断中的用途，以及检测hsa-miR-188-3p、hsa-miR-517a-3p、hsa-miR-518f-5p和hsa-miR-511-3p所用的试剂盒。
摘要（英）：

The invention belongs to the technical field of biology and in particular relates to a kit based on serum miRNA as well as a use method of the kit and application of the kit to early infection detection of fever with thrombocytopenia syndrome viruses. MiRNA molecules comprise hsa-miR-188-3p, hsa-miR-517a-3p, hsa-miR-518f-5p and hsa-miR-511-3p. The expression of the four miRNAs in early attack of the fever with thrombocytopenia syndrome viruses is specifically and remarkably increased through identification by adopting a Taqman low-density chip and a qRT-PCR (quantitative reverse transcription polymerase chain reaction) method. The four serum miRNAs are used as molecular targets, and a quick, sensitive and specific detection method for acute infection of the fever with thrombocytopenia syndrome viruses is built. The hsa-miR-188-3p, the hsa-miR-517a-3p, the hsa-miR-518f-5p and the hsa-miR-511-3p are applied to early diagnosis of fever with thrombocytopenia syndrome virus infection, and the kit is used for detecting the hsa-miR-188-3p, the hsa-miR-517a-3p, the hsa-miR-518f-5p and the hsa-miR-511-3p.

公开/授权文献
0/0