利索-全球专利检索

  1. 登录
  2. 注册
发明授权
CN104130988B 一种1,3-1,4-β-葡聚糖酶突变体 失效 - 权利终止 转让

一种1,3-1,4-β-葡聚糖酶突变体
摘要：
本发明公开了一种1,3-1,4-β-葡聚糖酶突变体，属于基因工程和酶工程领域。本发明将来源于特基拉芽孢杆菌(Bacillus terquilensis)的1,3-1,4-β-葡聚糖酶的第20位，117位和165位的赖氨酸通过重叠延伸PCR的方法突变为丝氨酸，分别获得单突变体K20S，K117S和K165S。将三个突变位点进行整合突变，获得K20S/K117S/K165S三突变酶。四株突变酶表现出了更高的催化活力及更好的热稳定性。这些突变酶和野生酶相比更有利于其在工业上的应用。
摘要（英）：

The invention discloses a 1,3-1,4-beta-glucanase mutant, and belongs to the fields of gene engineering and enzyme engineering. Lysine in the 20th position, 117th position and 165th position of 1,3-1,4-beta-glucanase from Bacillus terquilensis mutates through an overlapping extension PCR method to form serine in order to obtain single mutants K20S, K117S and K165S respectively. The above three mutation sites are integrally mutated to obtain a K20S/K117S/K165S triple mutant enzyme. Four mutant enzymes have higher catalysis activity and better thermal stability. Compared with wild enzymes, the above mutant enzymes are in favor of realizing the industrial application.

公开/授权文献
0/0