The invention belongs to the technical field of medicines, relates to an injection-use naloxone hydrochloride powder injection medicine composition and a preparation method thereof and particularly relates to the injection-use naloxone hydrochloride powder injection medicine composition which includes naloxone hydrochloride and especially includes the naloxone hydrochloride, a freeze-drying excipient and medicinal additives. The naloxone hydrochloride is added to the composition in the form of 17-allyl-4,5[alpha]-epoxy-3,14-dihydroxylmorphinan-6-one hydrochloride dihydrate. The freeze-drying excipient is selected from one or more of mannitol, sorbitol, lactose, maltose, glycine, trehalose, glucose and the like. The medicine composition can be used of post-operation of compound anesthesia with opioid medicines and has an antagonism effect of respiratory depression caused by the opioid medicines for promoting analepsia of a patient. The medicine composition is used in overdose of the opioid medicines, is used for reversing the respiratory depression caused by the opioid medicines completely or partially, is used for rescuing acute alcoholism and is used for diagnosis of acute opioid medicines overdose.