The invention discloses moxifloxacin hydrochloride in-situ gel ear drops. The ear drops are prepared from components such as moxifloxacin hydrochloride used as a main medicine ingredient, budesonide and ambroxol which are used as auxiliary medicines as well as carbomer, polyethylene glycol, menthol, borneol, boric acid and the like which are additionally added. The invention further discloses a preparation method of the moxifloxacin hydrochloride in-situ gel ear drops. The moxifloxacin hydrochloride is a broad-spectrum antibiotic of the fourth generation of quinolones, can perform sterilization effectively and is lasting in effect; the gel ear drops can act on affected parts directly, and the dosage is reduced, so that adverse reactions caused by whole body absorption are reduced, and the patient compliance is improved. The budesonide and the ambroxol are combined for use, so that inflammatory reactions and immunity suppression effects of mucosae can be reduced, discharge of otitis media hydrops and secretions is promoted, and the synergistic effect of eliminating etiologies and restoring functions is realized.