The present invention belongs to the technical field of optic fiber gyroscopes, and in particular relates to a preparation method of a sensing ring for inhibiting optic fiber gyroscope temperature drift. The method comprises: at the upper and lower sub-regions of the ring skeleton, adopting a quadrupole symmetry method to respectively wind a polarization maintaining optical fiber to form two optical fiber rings, wherein the number of the layers of the fiber rings in the upper sub-region is consistent with the number of the layers of the fiber rings in the lower sub-region, the winding number of each layer of the optical fiber in the upper sub-region is consistent with the winding number of each layer of the optical fiber in the lower sub-region, and the directions of the sensitive axis of the two optical fiber rings are opposite through observing from the axial direction of the ring skeleton; curing the optical fiber rings; and adjusting the gyroscope temperature drifts formed respectively by the two optical rings to make the amplitude be equal and make the direction be opposite. According to the present invention, the adverse effects of the temperature on the gyroscope performance in the prior art is inhibited, the zero drift of the gyroscope under the temperature change environment is reduced, the gyroscope precision is improved, and the engineering application level is improved.