The invention discloses a gene detection method of a miltenberger blood type Mur antigen. The method is suitable for detection of GP.Mur, GP.Bun, GP.HF and GP.Hop. The detection by using a loop-mediated isothermal amplification technology (LAMP) comprises the following steps: design of LAMP primers; extraction of blood genome DNA; target fragment and result judgment in amplification products of LAMP. In the manner, according to the gene detection method for detecting the miltenberger blood type Mur antigen disclosed by the invention, a loop-mediated isothermal amplification method is built by using designed primers; the reaction specificity is enhanced; the reaction time is shortened; reaction can be finished under an isothermal condition; the amplification products are macroscopic; and compared with a traditional PCR method, the method is more economical and practical, does not need special apparatus, and is simple in operation, and suitable for units such as hospitals, blood stations and the like, and convenient to popularize and use.