The invention discloses a Jingke 528 three-line mating hybrid seed production method. The hybrid seed production method provided by the invention comprises the following steps: using a maize sterile line S 90110-2 as a sterile line, a maize inbred line 90110-2 as a maintainer line and a maize inbred line Jing 2437 as a restorer line to carry out three-line mating seed production to obtain a hybrid Jingke 528. Experimental results show that Jingke 528 three-line mating hybrid seed production is carried out by utilizing the bred sterile line S 90110-2, the maintainer line 90110-2 and the restorer line Jing 528, and prepared sterile hybrid Jingke 528 seeds are fundamentally the same as Jingke 528 seeds produced by using a conventional seed production method in the aspects of yield, resistance and other agronomic characters.