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发明公开
CN104404038A 一种水稻对稻瘟病菌基础抗性的InDel分子标记及其应用 无效 - 驳回

一种水稻对稻瘟病菌基础抗性的InDel分子标记及其应用
摘要：
一种水稻对稻瘟病菌基础抗性的Indel分子标记及其应用，可用于稻瘟病菌基础抗性的鉴定。该Indel分子标记的核苷酸序列为SEQ ID No.1。利用本发明的引物对，以水稻基因组DNA为模板进行PCR扩增，可以得到与水稻对稻瘟病菌基础抗性基因紧密连锁的InDel分子标记，该标记在本发明中命名为InDelBR1。通过PCR扩增分析可以明确该标记的有无可以判断水稻对稻瘟病菌基础抗性的有无，能够用于水稻对稻瘟病菌基础抗性有无的检测。该InDel分子标记带型简单、分布较密等特点具有SSR不可比拟的优势，能快速精确检测亚种内品种间的多态性，具有广阔的应用前景。
摘要（英）：

The invention discloses an InDel molecular marker for basic resistance of rice to rice blast germs and application of the InDel molecular marker and the InDel molecular marker can be used for identifying the basic resistance to rice blast germs. The nucleotide sequence of the InDel molecular marker is shown in SEQ ID No.1. A primer pair of the InDel molecular marker can be adopted for PCR amplification by taking rice genome DNA as a template, the InDel molecular marker which is tightly linked with the basic resistance genes of rice to rice blast germs can be obtained, and the marker is named as InDelBR1 in the invention. Due to PCR amplification analysis, whether the rice has the basic resistance to the rice blast germs or not can be definitely judged, and whether the rice has the basic resistance to the rice blast germs or not can be detected. The InDel molecular marker has the characteristics of simple banding pattern, relatively dense distribution and the like, has unparallel advantages when being compared with SSR, is capable of rapidly and precisely detecting the polymorphism of subspecies, and has a wide application prospect.

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