The invention discloses a multi-target inhibiting base material for aquatic muscle endogenous enzyme and a preparation method and an application of the multi-target inhibiting base material. The multi-target inhibiting base material for aquatic muscle endogenous enzyme is prepared from a botanical food material composition prepared from cassava, potatoes, paddy rice and wheat. The multi-target inhibiting base material is characterized by comprising the following components in percentage by mass: 20-35% of cassava, 35%-50% potato, 10%-25% paddy rice and 10%-25% of wheat. Through reasonably proportioning and screening the obtained botanical food material, and through proper physical processing, the enzyme inhibiting base material similar to edible albumen powder is obtained, and can be applied to targeted inhibition of the aquatic muscle endogenous enzyme in aquatic product processing.