The invention discloses an enzymolysis method of ovalbumin, comprising the following steps: (1) ovalbumin, papaya peplidase, L-cysteine and distilled water are uniformly mixed to carry out an enzymolysis reaction, wherein amino acid sequence of papaya peplidase is sequence as shown in SEQ ID NO:1; (2) a mixture obtained after enzymolysis in the step (1) is heated, and ovalbumin residue which hasn't undergone enzymolysis is filtered and removed; and (3) enzymolysis products obtained after processing in the step (2) are sterilized. Simple, low-cost and high-purity extraction of papaya peplidase from papaya latex is realized. In addition, papaya peplidase is a natural biologically-active substance. By the use of the peptidase for enzymolysis of ovalbumin, low-molecular oligopeptides and amino acid with molecular weight being 500-1000 can be obtained. The enzymolysis rate is high. The low-molecular oligopeptides and amino acid can be used as medicines for treating hepatopathy and also can further promote absorption of organisms and skin.