The invention relates to the field of gene detection and particularly relates to a nucleic acid membrane strip and a kit for detecting a gene of hereditary hearing loss. The nucleic acid membrane strip comprises a substrate and specific oligonucleotide probes which are fixed on the substrate, wherein the sequences of the oligonucleotide probes are shown in SEQ ID NO: 1-42. According to another technical scheme, the invention provides a kit for detecting the gene of hereditary hearing loss. The kit comprises the nucleic acid membrane strip detecting the gene of hereditary hearing loss, a PCR reaction liquid I and a PCR reaction liquid II. The nucleic acid membrane strip and kit provided by the invention have the beneficial effects that the nucleic acid membrane strip and kit comprise eight pairs of primers capable of being efficiently and specifically amplified and 42 probes capable of being specifically hybridized. The detection sites are relatively complete, so that 21 common hearing loss sites can be simultaneously detected by using two tubes of the reaction liquid and one membrane strip.