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发明公开
CN104611400A 一种辅酶再生与树脂原位萃取促进ColletotrichumliniST-1对DHEA的羟化方法 无效 - 驳回

一种辅酶再生与树脂原位萃取促进ColletotrichumliniST-1对DHEA的羟化方法
摘要：
本发明属生物技术领域，涉及一种利用辅酶再生与树脂原位萃取“二合一”的策略促进亚麻刺盘孢霉（Colletotrichum lini）ST-1对去氢表雄酮（DHEA）的羟化。其特征在于，转化过程中加入10 g/L的辅底物进行NADPH再生，同时利用D101型大孔吸附树脂原位萃取产物，从而显著提高C. lini ST-1对DHEA的转化率。在底物投料量为10 g/L的条件下，底物DHEA的转化率为93%，7α,15α-diOH-DHEA的产量可达到为7.12 g/L。
摘要（英）：

Belonging to the field of biotechnology, the invention relates to a method of utilizing a coenzyme regeneration and resin in-situ extraction integrated strategy to promote hydroxylation of DHEA (dehydroepiandrosterone) by Colletotrichum lini ST-1. The method is characterized in that in the conversion process, a 10g/L cosubstrate is added to conduct NADPH regeneration, and simultaneously D101 type macroporous adsorption resin is employed for in-situ extraction of the product, thus significantly enhancing the conversion rate of C. lini ST-1 on DHEA. Under the condition of a substrate feeding amount of 10g/L, the conversion rate of the substrate DHEA is 93%, and the yield of 7 alpha, 15 alpha-diOH-DHEA can reach 7.12 g/L.

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