The invention relates to a chicken infectious bronchitis live vaccine potency detection method, which comprises the following steps: 1) designing a primer; 2) diluting, inoculating and cultivating virus; 3) harvesting virus; 4) performing PCR detection; and 5) calculating virulent valence. The chicken infectious bronchitis live vaccine potency detection method is simple to operate, uniform in determination conditions, strong in controllability, small in batch errors, the detection period of the chicken infectious bronchitis virus potency is shortened, and the application range is wide.