The invention discloses pseudochrobactrum asaccharolyticum WZZ003 and application thereof. The application comprises split preparation of (R)-2-(2, 6-dimethyl phenyl amino) propionic acid under the condition that the temperature is 20-50 DEG C and the pressure is 200rpm by taking a wet thallus obtained by fermenting culture of pseudochrobactrum asaccharolyticum WZZ003 or a dried thallus obtained by freezing and drying the wet thallus as a catalyst, racemized (R, S)-2-(2, 6-dimethyl phenyl amino) methyl propionate as a substrate and a phosphate buffer with the pH value of 5.0-7.2 as a reaction medium. An enzyme-producing microbial bacterial strain disclosed by the invention: pseudochrobactrum asaccharolyticum WZZ003 is strong in region selectivity, and the enantiomeric excess value of the prepared product is greater than or equal to 99.5%, the reaction conversion rate is high, the conversion rate reaches over 99% through recycle of byprodcuts, and the application is simple in downstream separation, low in energy consumption, small in environmental pollution and suitable for industrial production.