The invention discloses dehydroabietylamine imidazole organic salt, and a preparation method and application thereof, and belongs to a dehydroabietylamine-based compound. The dehydroabietylamine imidazole organic salt is 4,5-imidazole diformate mono-dehydroabietylamine organic salt. The preparation method comprises the following steps: reacting dehydroabietylamine with 4,5-imidazole dicarboxylic acid under a methylbenzene medium for 8 to 12h, wherein the mole ratio of the dehydroabietylamine to the 4,5-imidazole dicarboxylic acid is 1:1.0 to 1:1.5; after the reaction is finished, performing filtering, and rotationally evaporating methylbenzene to obtain the compound. The compound can also be applied to antitumor drugs and shows high anti-cervical cancer activity. The preparation method is easy to operate, and items needed in an experiment are low in toxicity.