The invention discloses a method for determining allowable fatigue stress range of a high-strength steel bar base material and a connection structure of the base material. The method comprises: according to the types of the high-strength steel bar base material and the connection structure of the base material, determining a basic allowable fatigue stress range; and obtaining measurement parameters of the high-strength steel bar base material and the connection structure of the base material, and according to obtained industrial parameters, selecting a corresponding calculation coefficient from a preset corresponding relation table. With the adoption of the method for determining the allowable fatigue stress range of the high-strength steel bar base material and the connection structure of the base material, the allowable fatigue stress range of the high-strength steel bar base material and the connection structure of the base material can be determined accurately.