The invention relates to a glucoamylase fermentation process for utilizing a fermentation broth partition method to improve fermentation tank use efficiency and belongs to the technical field of enzyme engineering and fermentation engineering. At the terminal stage of fermenting enzyme activity linear growth of aspergillus niger GB0506 glucoamylase, fermentation broth is distributed to several unused fermentation tanks, a fresh fermentation medium is added to go on with the fermentation, the best opportunity of fermentation broth partitioning is at fermentation for 118-120 h, and the optimal partitioning mode is that one tank is partitioned into three tanks. Compared with the prior art, the final fermenting enzyme activity of the fermentation broth obtained through the process has no obvious changes, the average in-tank fermentation time of each fermentation tank is greatly reduced, and the electric energy consumption in the fermentation process is greatly reduced. The invention further provides a fermentation tank combination use method for averagely distributing the fermentation broth to three fermentation tanks. On the corresponding process conditions, the fermentation strength of unit fermentation volume is improved by over 54%. The novel glucoamylase fermentation process by means of the fermentation broth partition method greatly improves fermentation tank use efficiency for enzyme preparation production enterprises.