The invention discloses a hybridization probe for detecting von hippel-lindau (VHL) gene large deletion and a detection method and a kit, which relate to the field of gene detection, wherein the hybridization probe is marked with fluorescence signals, and is prepared from probe primers with nucleotide sequences which is showed in sequence identifier number 1-6 (SEQ ID NO.1-6). The detection method comprises steps: cell smears are prepared from collected samples to be detected, hybridization probe is arranged in a hybridization solution to have a hybridization reaction with cell and obtain hybridization products, the hybridization products are washed, dyeing of cell nucleus are processed to obtain cell smears after nuclear staining, the cell smears after nuclear staining are observed through a fluorescence microscope, thereby judging whether deoxyribose nucleic acids (DNA) of the samples to be detected happen VHL gene large deletion. The kit comprises the hybridization probe. The hybridization probe is strong in specificity, high in sensitivity, simple in detection method and low in cost of the kit, and can rapidly and accurately detect VHL gene large deletion.