The invention provides a multi-satellite separation parameter optimization method which comprises the steps of a first step, determining an equivalent objective function and a parameter set according to a multi-satellite separation main object requirement; a second step, performing sensitivity analysis on the parameter set according to the objective function for obtaining a key parameter set which is composed of key parameters; a third step, calculating an optimal solution of the key parameter set according to the objective function; a fourth step, performing a track simulation test for screening an easy collision flying object set; and a fifth step, applying an interference with specific frequency distribution on the key parameter set, performing a Monte Carlo simulation test, calculating a warning probability and collision probability of each satellite in the easy collision flying object set, if the warning or collision probability is larger than or equal with a respective preset maximal value, adjusting a weighting coefficient in the objective function and returning to the step 3, and if the warning or collision probability is smaller than a respective preset maximal value, outputting the warning probability, the collision probability and the corresponding optimal key parameter set.