The invention provides a formula and a preparation method for a gel support used for in-vitro construction of tissue-engineered cartilage. The formula comprises gel and an additive, wherein the gel may be fibrin, collagen, hyaluronic acid and thrombin, and the additive may be vitamin C, linoleic acid, cholesterol, dexamethasone, transferrin, biotin and the like. With the formula and the support prepared by using the method, cartilage cells cultured in vitro maintain activity of original cells; and the gel support with plasticity can be provided according to the shape of a cartilage defect of a patient in clinical application of cartilage tissue engineering.