The invention relates to a method for detecting trichlorfon chiral enantiomers in aquatic products. The method is characterized by including the following steps that firstly, fish tissue is weighed and minced and placed in a centrifugal machine for centrifugal separation after tissue homogenate and continuous homogenization and stands still, and supernate is obtained for use after standing; secondly, the supernate in the first step is processed with anhydrous sodium sulfate, passes C18 and PSA serial connection columns, an acetonitrile solution is added after flowing of the supernate is completed for leaching, the supernate is collected to a volumetric flask, nitrogen is blown till the supernate is nearly dried, a mobile phase of normal hexane and isopropanol is used for setting the volume to be 2 mL, the supernate passes a 0.22 organic filtering membrane to obtain a solution A to be detected, and the size proportion of normal hexane and isopropanol of the mobile phase is 85-95 to 15-5; thirdly, an efficient liquid phase chromatographic instrument is used for detecting the solution A, a CHIRALCEL IC chiral column is selected in detection, the column temperature of the chiral column is 20-35 DEEG C, the flow speed during detection is 0.7 mL/min-1.0 mL/min, and the wavelength is 207 nm. By means of the method, resolution of the trichlorfon chiral enantiomers can be successfully achieved, and the residual quantity of the trichlorfon chiral enantiomers in the aquatic products is detected.