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发明授权
CN105349555B 一种人工合成的Bt抗虫基因FLIa及其制备方法和应用 有权

一种人工合成的Bt抗虫基因FLIa及其制备方法和应用
摘要：
一种人工合成的Bt抗虫基因FLIa及其制备方法和应用，属于抗虫蛋白及生物安全技术领域。该Bt抗虫基因FLIa的核苷酸序列如SEQ ID NO:1所示，其编码的蛋白的氨基酸序列如序列表中的SEQ ID NO:2所示。该Bt抗虫基因FLIa的制备方法为：将抗虫基因Cry1Ab的DomainⅠ和DomainⅡ与抗虫基因Cry1Ia的DomainⅢ进行交换融合，获得重组抗虫基因MCry1I；在重组抗虫基因MCry1I的基础上，在3’端连入抗虫基因Cry1Ja1的碳末端，得到重组抗虫基因FLMCry1Ia；对重组抗虫基因FLMCry1Ia进行密码子改造，获得Bt抗虫基因FLIa。该Bt抗虫基因FLIa可在转化植物中稳定遗传和表达，且表达量高，所得转基因植物抗虫性好。该基因转化玉米、棉花、水稻、蔬菜等农作物，使其具备相应的抗虫活性，从而降低农药的使用量，以减少环境污染和生产成本。
摘要（英）：

The invention provides an artificially synthesized Bt insecticidal gene FLIa as well as a preparation method and application thereof, belonging to the technical field of insecticidal protein and biosafety. The nucleotide sequence of the Bt insecticidal gene FLIa is shown as SEQ ID NO: 1, and the nucleotide sequence of encoded protein of the Bt insecticidal gene FLIa is shown as SEQ ID NO: 2 in a sequence table. The preparation method of the Bt insecticidal gene FLIa comprises the steps of exchanging and fusing Domain I and Domain II of a insecticidal gene CrylAb and Domian III of a insecticidal gene CrylIa to obtain a recombinant insecticidal gene MCrylI; on the basis of the recombinant insecticidal gene MCrylI, connecting the carbon terminal of a insecticidal gene CrylJal at a 3minute terminal to obtain a recombinant insecticidal gene FLMCrylIa; performing codon reconstruction on the recombinant insecticidal gene FLMCrylIa to obtain the Bt insecticidal gene FLIa. The Bt insecticidal gene FLIa can be stably inherited and expressed in transformation plants, and is high in expression quantity, and obtained transgenic plants are good in insect resistance. The gene is used for transforming crops of corn, cotton, rice, vegetables and the like, and enabling the same to have corresponding insect-resistant activity, so that the using amount of pesticides is reduced and environment pollution and production cost are reduced.

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