The invention relates to a copper ions detection method based on nano-cluster. The method is characterized by comprising the following steps: 1) mixing a standard copper ions solution and gold nanoclusters, establishing a standard curve of fluorescence signal quenching strength for detecting the nano-cluster and copper ions amount; and 2) mixing a sample solution and the gold nanoclusters, bring the fluorescence signal quenching strength for detecting the nano-cluster into the standard curve to obtain the content of copper ions in the sample. The concentration of the gold nanoclusters is 0.5 mM, a pH value is 7; and the gold nanoclusters are coated and modified by GSH. The experiment proves that the sensitivity of the detection method is high, detection interference of other ions to the copper ions is less, the method can realize selectivity detection to the copper ions, so that a purpose for rapidly detecting the concentration of copper ions can be reached, and the method has wide application prospect for copper ion detection.