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发明公开
CN105385759A 尼罗罗非鱼种质纯度鉴定用引物及PCR鉴定方法 无效 - 驳回

尼罗罗非鱼种质纯度鉴定用引物及PCR鉴定方法
摘要：
本发明公开了一种尼罗罗非鱼种质纯度鉴定用引物及PCR鉴定方法，所述引物能够对尼罗罗非鱼的MyoD基因进行扩增，生成一条229bp的特异性扩增片段。(1)使用本发明的引物对尼罗罗非鱼的基因组DNA进行扩增，生成一条229bp的特异性扩增片段，与奥利亚罗非鱼的1条264bp条带，奥尼罗非鱼的2条分别为229bp和264bp的条带，能够明显区分，因而可以简单、精确地对尼罗罗非鱼的种质纯度进行鉴定；(2)本发明所述引物特异性强，不会对目标片段以外的其它片段扩增；(3)本发明公开了使用所述引物的PCR鉴定方法，该方法操作快捷、结果准确，避免了采用传统形态学方法鉴定不同品种的罗非鱼带来的结果不稳定的缺陷。
摘要（英）：

The invention discloses a primer for nile tilapia germ plasm purity identification and a PCR identification method. The primer is capable of amplifying an MyoD (Myogenic Differentiation Antigen) gene of nile tilapia, so as to generate a strip of 229bp specific amplification fragment. (1), the primer provided by the invention is used to amplify the genomic DNA of the nile tilapia, so as to generate the strip of 229bp specific amplification fragment, and a strip of 264bp band of blue tilapia, wherein two strips of hybrid tilapia are respectively 229bp and 264bp bands, which can be obviously distinguished, and thus the germ plasm purity identification of the nile tilapia can be simply and accurately carried out; (2), the specificity of the primer provided by the invention is high, and other fragments except the target fragment cannot be amplified; (3), the invention discloses the PCR identification method using the primer, and the method is fast and convenient in operation and accurate in result, so that the defect of unstable result brought about by adopting a conventional morphological method to identify the nile tilapia of different species is avoided.

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