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CN105441467A 一种嗜热碱性果胶裂解酶基因、工程菌、嗜热碱性果胶裂解酶及其应用 失效 - 权利终止

  • 专利标题： 一种嗜热碱性果胶裂解酶基因、工程菌、嗜热碱性果胶裂解酶及其应用
  • 专利标题（英）： Thermophilic alkaline pectin lyase gene, engineering bacterium, thermophilic alkaline pectin lyase and application thereof
  • 申请号： CN201510901096.3
    申请日： 2015-12-09
  • 公开(公告)号： CN105441467A
    公开(公告)日： 2016-03-30
  • 发明人: 张作明 , 李海超 , 张静 , 李正强 , 于擎
  • 申请人： 吉林大学
  • 申请人地址： 吉林省长春市前进大街2699号
  • 专利权人： 吉林大学
  • 当前专利权人： 吉林大学
  • 当前专利权人地址： 吉林省长春市前进大街2699号
  • 代理机构： 长春吉大专利代理有限责任公司
  • 代理商 王淑秋; 王恩远
  • 生物保藏信息： CGMCC No.11460 20150929
  • 主分类号： C12N15/60
  • IPC分类号： C12N15/60 ;  C12N9/88 ;  C12N1/21 ;  C12N15/70 ;  C12R1/19
一种嗜热碱性果胶裂解酶基因、工程菌、嗜热碱性果胶裂解酶及其应用
摘要：
一种嗜热碱性果胶裂解酶基因、工程菌、嗜热碱性果胶裂解酶及其应用，属于生物工程技术领域。该工程菌的保藏编号为CGMCC No.11460，分类命名为大肠埃希氏菌Escherichia coli，保藏单位为中国微生物菌种保藏管理委员会普通微生物中心，保藏日期为2015年9月29日。本发明主要公开嗜热碱性果胶裂解酶，最适反应温度为70℃，最适Ca2+浓度为0.8mM，底物为0.2％(w/v)多聚半乳糖醛酸，pH9.5。该酶在最适pH9.5、Ca2+0.8mM、70℃时酶比活为900U/mg；在70℃保温下，半衰期为6h；经过镍柱纯化处理后CbPelC可达到50mg/L(1L发酵液)。本发明所述嗜热碱性果胶裂解酶的耐热性和产量有大幅度挺高，更加适应现代工业生产要求，也为工业大规模生产碱性果胶酶提供了有效地方法。
摘要（英）：

The invention provides a thermophilic alkaline pectin lyase gene, an engineering bacterium, thermophilic alkaline pectin lyase and application thereof and belongs to the technical field of biological engineering. The preservation number of the engineering bacterium is CGMCC No.11460, the engineering bacterium is named as Escherichia coli through classification, the depository authority is the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the preservation date is September 29, 2015. The invention mainly discloses the thermophilic alkaline pectin lyase, the optimum reaction temperature is 70 DEG C, the optimal Ca2+ concentration is 0.8 mM, a substrate is 0.2% (w/v) polygalacturonic acid, and pH is 9.5. The specific activity of the lyase is 900 U/mg under the optimal conditions that the pH is 9.5, the Ca2+ concentration is 0.8 mM and the temperature is 70 DEG C; heat preservation is performed at the temperature of 70 DEG C, and a half-life period is 6 h; after nickel column purification treatment is performed, CbPelC can be up to 50 mg/L (1 L fermented liquid). The heat resistance and yield of the thermophilic alkaline pectin lyase are remarkably improved, the thermophilic alkaline pectin lyase more adapts to the modern industrial production requirements, and an effective method is provided for industrial mass production of the alkaline pectinase.

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