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CN105441470A 一种检测植物自噬现象的基因定位表达载体及应用 无效 - 驳回

一种检测植物自噬现象的基因定位表达载体及应用
摘要：
本发明属于生物技术领域，具体涉及检测细胞自噬现象的基因载体的构建。应用本发明设计的Atg8基因定位表达载体，使其蛋白与GFP的蛋白融合后在悬浮细胞中表达，通过激光共聚焦显微镜检测融合蛋白的位置而进行的亚细胞定位，是一种简便而又准确的在活细胞中进行定位的方法，为研究烟草细胞自噬现象的分子机理提供参考依据。
摘要（英）：

The invention belongs to the technical field of biology, and particularly relates to construction of a gene vector for detecting a cell autophagy phenomenon. A protein of an Atg8 gene mapping expression vector is expressed in a suspension cell after being fused with a green fluorescence protein (GFP), and subcellular localization is performed by detecting the position of a fused protein through a laser scanning confocal microscope. Through a simple, convenient and accurate method for locating in living cells, a reference basis is laid for the research of the molecular mechanism of the tobacco cell autophagy phenomenon.

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