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发明公开
CN105462949A 一种谷氨酸脱羧酶突变体及其制备方法和用途 无效 - 驳回

一种谷氨酸脱羧酶突变体及其制备方法和用途
摘要：
本发明公开了一种谷氨酸脱羧酶突变体及其制备方法和用途，该突变体的氨基酸序列如SEQ ID NO.2所示，核苷酸序列如SEQ ID NO.1所示。本发明还公开了一种包含编码谷氨酸脱羧酶突变体基因的表达单元、重组质粒和转化子。本发明根据与嗜热古细菌(Thermococcus kodakarensis)GAD氨基酸序列的比对信息，采用定点突变的方法在短乳杆菌GAD对应的氨基酸位点引入脯氨酸残基，通过理性设计提高GAD的热稳定性，该突变酶在催化L-谷氨酸或其钠盐生成γ-氨基丁酸的过程中具有更好的热稳定性，有利于GABA的工业化生产。
摘要（英）：

The invention discloses a glutamic acid decarboxylase mutant and a preparation method thereof and application. An amino acid sequence of the mutant is as shown in SEQ ID No. 2, and a nucleotide sequence is as shown in SEQ ID No. 1. The invention further discloses an expression unit containing genes for encoding the glutamic acid decarboxylase mutant, a recombinant plasmid and a transformant. According to information of comparison with a thermococcus kodakarensis glutamic acid decarboxylase (GAD) amino acid sequence, a method of site-directed mutagenesis is adopted to introduce proline residue at an amino acid locus corresponding to lactobacillus brevis GAD, and the thermal stability of the GAD is improved through rational design. The mutant has better thermal stability in the process of catalyzing L-glutamic acid or sodium salts thereof to generate gamma-aminobutyric acid and is favorable for industrial production of gamma amino acid butyric acid (GABA).

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