The invention discloses Antarctic krill freshness seasoning based on a Maillard reaction and a preparation method thereof. The preparation method includes the steps that raw materials are cleaned, shells are removed to pick meat, then thermal degradation is conducted, biological enzyme hydrolysis is conducted twice, and Antarctic krill protein is fully degraded; then the Maillard reaction and a lipid fragrance generation reaction are conducted, in the reaction processes, reducing sugar, amino acid, edible oil and vitamin are added and heated for a complete reaction, and Maillard reaction liquid is obtained; finally, seasoning and sterilizing are conducted, the Antarctic krill freshness seasoning in the liquid form is obtained, or spray drying is conducted after excipient is added, and the Antarctic krill freshness seasoning in the solid form is obtained. The seasoning prepared through the method has the advantages of being pure in taste and heavy and mellow in krill fragrance; the used biocatalysts are all protease obtained through food-grade microorganism fermentation and are high in safety; various used raw materials are from natural matter, do not contain chemical addition agents and have relatively high safety, and comprehensive nutrition is enhanced.