The invention belongs to the technical field of biomolecules, and relates to a rapid sensitivity detection technology, in particular to a duplex-PCR detection method for maize kernel rot pathogen. The method comprises the specific steps of 1, collection of hyphae; 2, extraction of fungus DNA; 3, PCR amplification, wherein a PCR reaction total system comprises 1 microliter of a fusarium graminearum primer FvCal58-F/FvCal240-R, 1 microliter of a Verticillium Fusarium primer Fg16N-F/Fg16N-R, 5 microliters of 2*r-taq Master mix, 1 microliter of template DNA and 10 microliters of sterilization double distilled water in a supplementing mode; the duplex PCR reaction procedure comprises the steps of performing initial denaturation at the temperature of 94 DEG C for 5 min, performing denaturation at the temperature of 94 DEG C for 30 s, performing annealing at the temperature of 54 DEG C for 30 s, performing extending at the temperature of 72 DEG C for 20 s, performing 35 cycles and performing extending at the temperature of 72 DEG C for 10 min. The simultaneous detection method established by means of duplex PCR is economical, the laboratory time and detection cost are greatly saved, the method can be applied to detection in actual production, a foundation is laid for rapid detection for maize kernel rot pathogen caused by a field, and maize kernel rot can be prevented and treated in advance.