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发明公开
CN105567722A 一种丁酸产量低且丁醇产量高的工程菌及其构建方法与应用 有权

一种丁酸产量低且丁醇产量高的工程菌及其构建方法与应用
摘要：
本发明公开了一种丁酸产量低且丁醇产量高的工程菌及其构建方法与应用。本发明提供了一种构建重组菌的方法，为抑制或沉默生产丁醇的出发菌的基因组上的硫酯酶基因yciA表达，得到重组菌。本发明通过将生产丁醇的出发菌EB228CGMCC No.11986基因组上的硫酯酶YciA基因yciA替换为FRT，得到重组菌，该重组菌发酵产生的丁酸产量明显下降，丁醇产量有较明显的上升，可以用于丁醇高产量生产。
摘要（英）：

The invention discloses an engineering bacterium low in butyric acid yield and high in butyl alcohol yield and a construction method and application thereof. The invention provides the method for constructing recombinant bacteria to restrain or silence expression of a thioesterase gene yciA on a genome of an original strain for producing butyl alcohol and obtain a recombinant bacterium. The recombinant bacterium is obtained by replacing the thioesterase gene yciA on the genome of the original strain EB228CGMCC No.11986 for producing butyl alcohol with FRT. The yield of butyric acid generated by the recombinant bacterium through fermentation is obviously decreased, the yield of butyl alcohol generated by the recombinant bacterium through fermentation is obviously increased, and the engineering bacterium can be used for high-yield production of butyl alcohol.

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