The invention belongs to the technical field of high-valued utilization of euphausia superba, and relates to seasoning for seafood and a preparation method thereof, in particular to seasoning for the euphausia superba and the preparation method thereof. The preparation method comprises the following steps of: adopting the euphausia superba as a raw material, adding an enzyme preparation for enzyme control and enzymolysis after cleaning, shelling to take out euphausia superba meat and homogenizing, adding salt, flavor disodium nucleotide, ginger powder, caramel colour and hydroxy propyl distarch phosphate according to the proportion after enzyme inactivation, mixing to prepare seasoning liquid, carrying out bottled package, and sterilizing to obtain the liquid seasoning for the seafood. The finished seasoning has the advantages that rich nutrient substances such as protein, amino acid, polyunsaturated fatty acid and astaxanthin and the like in euphausiid are reserved, nutrient elements such as the Omega-3 polyunsaturated fatty acid and the like can be supplemented by frequently eating the seasoning, so that the market prospect is wide.