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发明公开
CN105647513A 一种具有pH响应的双模式成像探针及其制备方法和应用 有权

一种具有pH响应的双模式成像探针及其制备方法和应用
摘要：
本发明提供了一种具有pH响应的双模式成像探针，化学结构式如式(Ⅰ)所示：式中，X为-C(CH3)2-、-O-、-S-或-Se-，Y为F、Cl、Br或I，R1和R2分别独立地选自H原子、C1-18烷基或-SO3R5，R5为C1-18烷基或苄基，R3和R4分别独立地选自C1-18烷基或苄基。该探针在pH3.5～10.0时，其在激发光760nm、670nm下的荧光强度比随着pH的降低而逐渐增强；在760nm、680nm处的吸收强度比随着pH的降低而逐渐增强，760nm/680nm光声信号随着pH降低而逐渐增强，表现出明显的pH依赖的光声信号变化性；且pKa＝6.2，很适合在荧光-光声的双模式下进行肿瘤pH的检测。本发明还提供了该探针的制备方法及应用。
摘要（英）：

The invention provides a dual-mode imaging probe with pH response. The chemical structural formula of the dual-mode imaging probe is as shown in the formula (I). In the formula (I), X represents -C(CH3)2-, -O-, -S- or -Se-; Y represents F, Cl, Br or I; R1 and R2 are independently selected from H atom, C1-18 alkyl or -SO3R5; R5 represents C1-18 alkyl or benzyl; R3 and R4 are independently selected from C1-18 alkyl or benzyl. When a pH of the probe is 3.5-10.0, the fluorescence intensity ratio of emission light at 760nm and 670nm is gradually enhanced along with the reduction of the pH; the absorption intensity ratio at 760nm and 680nm is gradually enhanced along with the reduction of the pH; 760nm/680nm photo-acoustic signals are gradually enhanced along with the reduction of pH; the obvious pH-depended photo-acoustic signal variability is shown; pKa is 6.2. The dual-mode imaging probe is very applicable to detection of tumor pH I in both a fluorescence mode and a photo-acoustic mode. The invention also provides a preparation method and application of the probe.

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