The invention discloses a bioassay method applicable to high-throughput chemical screening and a bioassay spray tower.The bioassay method has the advantages that owing to small spraying spatial distribution volume of spray heads, loss rate of chemicals due to dispersion of droplets in air is decreased, non-uniformity in spraying due to time difference between starting a driving mechanism and starting the spray heads is avoided, chemical spraying quantity is controlled precisely, accuracy of the bioassay method is improved remarkably, chemical consumption is reduced, and accordingly the bioassay method is particularly applicable to high-throughput chemical screening.The bioassay spray tower is novel in structural design, saving in space, easy to produce and manufacture and worthy of being popularized and used.