The invention relates to an application of a paddy rice temperature sensitive male sterility gene TMS10 and a fertility restoration method. The amino acid sequence of TMS10 is represented by SEQ ID No.1. A conventional method is adopted to knock, change or inhibit TMS10 gene to reduce the expression level of TMS10 gene in common paddy rice species so as to obtain paddy rice male sterility line. The prepared paddy rice sterility line does not have abnormal phenotype during the vegetative growth phase; anther development is abnormal during the reproductive stage; the expressions of the phenotype are the same in the mutants of non-glutinous rice and japonica rice; the paddy rice is sterile at an average temperature of 28 DEG C, and paddy rice is fertile at an average temperature of 22 DEG C. The temperature sensitive characteristic, which is utilized during the process of two line hybrid seed production, is widely used in agricultural production.