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发明公开
CN105950713A 鉴定啤酒中微生物种的方法及其在鉴定啤酒污染菌中的应用 审中-实审

鉴定啤酒中微生物种的方法及其在鉴定啤酒污染菌中的应用
摘要：
本发明公开了一种鉴定啤酒中微生物种的方法及其在鉴定啤酒污染菌中的应用。本发明提供了一种鉴定或辅助鉴定啤酒中微生物种的方法，包括(1)根据为微生物纯培养物的形态特征和生理生化特性进行分类鉴定，获得种确定的微生物纯培养物和/或种不确定的微生物纯培养物；所述生理生化特性不包括微生物碳源呼吸代谢；(2)根据微生物碳源呼吸代谢的差异对步骤(1)中所获得的种不确定的微生物纯培养物进行分类鉴定，得到种确定的微生物纯培养物和/或种不确定的微生物纯培养物；(3)利用PCR检测方法对步骤(2)中所获得的种不确定的微生物纯培养物进行分类鉴定，确定所述种不确定的微生物纯培养物的种。
摘要（英）：

The invention discloses a method for identification of microbial species in beer and an application thereof in identification of beer contaminant bacteria. The invention provides the method for identification or assisted identification of the microbial species in the beer, wherein the method includes the steps: (1) classifying and identifying according to morphological characteristics and physiological and biochemical characteristics of a microbial pure culture material, to obtain a species-determined microbial pure culture material and/or a species-undetermined microbial pure culture material, wherein the physiological and biochemical characteristics do not include microbial carbon source respiratory metabolism; (2) according to the difference of microbial carbon source respiratory metabolism, classifying and identifying the species-undetermined microbial pure culture material obtained in the step (1), to obtain a species-determined microbial pure culture material and/or a species-undetermined microbial pure culture material; and (3) classifying and identifying the species-undetermined microbial pure culture material obtained in the step (2) by a PCR detection method, and determining the species of the species-undetermined microbial pure culture material.

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