The invention relates to the technical field of pharmaceutical analysis, in particular to a method for measuring content of sodium calcium edetate in a medicine and more specifically relates to a method for measuring the content of the sodium calcium edetate in Cleviprex fat emulsion injection. According to the method, liquid chromatogram is used for detecting the sodium calcium edentate; before detection, pre-column derivatization is carried out on the sodium calcium edetate so that the sodium calcium edetate can be converted into sodium ion EDTA; then, spherical graphite carbon serves as filler of a chromatographic column, and a mixed solution of a ferric sulfate water solution and ethanediol serves as a mobile phase for detection, wherein the pH of the mixed solution is 1-2. When the method is adopted for detection, main peak trailing factors are optimal, the number of theoretical plates is large, the peak width is small, symmetry is good, and the content of the sodium calcium edetate in the medicine can be precisely measured.