The invention provides a pseudorandom sequence generator based on PUF. The pseudorandom sequence generator comprises an entropy extracting module based on the PUF, an instantiation module, a reseeding module and a pseudorandom sequence generating module. A true random number is generated in a PUF mode to serve as entropy input, and pseudorandom sequences are generated by selecting different PUF implementation methods according to the characteristics of the pseudorandom sequences needing to be generated. When the bits of the sequences cannot meet the requirement, the generator can continue conducting iterative operation by generating a reseeding variable V' and a reseeding constant C' until the bits of the generated pseudorandom sequences meet the requirement. According to the pseudorandom sequence generator based on the PUF, the characteristic that the PUF cannot be cloned is fully utilized, and the pseudorandom sequences are generated by extracting the true random number as a seed through the irreversibility of a Hash algorithm. Compared with an existing pseudorandom sequence generator, the pseudorandom sequences generated by the pseudorandom sequence generator are low in cost and high in randomness and safety.