Provided is a method for preparing a nano anticancer probe of a zirconium-porphyrin metal organic framework material. Anhydrous zirconium chloride, meso-4-carboxyphenyl porphyrin, cetyl trimethyl ammonium bromide, benzoic acid and polyethylene glycol are added into N,N-dimethylformamide for ultrasonic dissolving, and an obtained mixed solution is subjected to a heating reaction till purple sediment is precipitated out at the bottom; after centrifugal separation, the sediment is washed with N,N-dimethylformamide, ethyl alcohol and water, and freeze drying is carried out to obtain a nano anticancer probe 1; chemotherapeutic medicine is dissolved in a 4-hydroxyethylpiperazine ethane sulfonic acid buffer solution, the nano anticancer probe 1 is added, centrifugal separation is carried out after stirring is carried out at normal temperature, washing is carried with a 4-hydroxyethylpiperazine ethane sulfonic acid buffer solution, freeze drying is carried out, and a nano anticancer probe 2 is obtained. The method has the advantages that the process is simple, and implementation is easy; the nano anticancer probe is used as an imaging induced synergic treatment system in the field of biomedicine, and meanwhile cancer is detected and treated.