The invention discloses a testing system for shear strength of metal and glass glue. The testing system comprises metal plates, a glass panel, clamp plates and a universal testing machine, wherein the universal testing machine is equipped with an upper clamping system and a lower clamping system; the two metal plates are clamped and fixed by the upper clamping system and the lower clamping system respectively, the upper metal plate and the lower metal plate are coaxially arranged, and a gap is formed between the upper metal plate and the lower metal plate; one side of the glass panel is fixedly connected with the upper metal plate and the lower metal plate through glue, the two clamp plates are arranged on the front side and the rear side of the glass panel respectively and are fixedly connected through multiple clamp plate connecting assemblies, and a glass positioning assembly propped against the glass panel is arranged on each clamp plate. When the testing system is in use, the glass panels of different lengths and different thicknesses are fixed conveniently, and the condition of unreasonable shear stress caused by lateral deviation of the glue or brittle fracture of a glass panel during late loading can be avoided.