The invention relates to a method for preparing a recombinant glycine-arginine insulin crystal. The method specifically comprises the following steps of (1) dissolving recombinant glycine-arginine insulin, a phenol derivative, a zinc-containing substance and organic acid in water so as to obtain crystal fluid; and (2) taking a pH regulating agent for regulating the pH value of the crystal fluid to 4.5-7.0, performing stirring for 3-9 hours, and performing separation so as to obtain the recombinant glycine-arginine insulin crystal, wherein the concentration of the recombinant glycine-arginine insulin in the crystal fluid is 1-3.5g/L, the mass/volume percentage of the phenol derivative is 0.01-1.00%, the mass/volume percentage of the zinc-containing substance is 0.005-1.5%, and the mass/volume percentage of the organic acid is 0.01-2.0%. The crystal fluid prepared by the method does not contain organic solvents, the operation process is safe, the technology is simple and easy to control, poisonous and harmful substances in chromatography in the previous step can be effectively removed, the time for filtration, collection, crystal washing and freeze drying of products is shortened, the cost is reduced, and the method is suitable for industrialized production of the recombinant glycine-arginine insulin.