The invention relates to a novel multichannel one-hole endoscope puncture outfit. The novel multichannel one-hole endoscope puncture outfit comprises a multi-hole operation platform and a channel pipe connected with the multi-hole operation platform. The channel pipe comprises a tubular flexible film channel and a lower ring arranged at the lower end of the flexible film channel, the upper end of the flexible film channel is connected with the multi-hole operation platform, and the multi-hole operation platform and the flexible film channel are connected through a sealing connection assembly. The sealing connection assembly comprises a flexible flange plate arranged at the upper end of the flexible film channel in a sleeving mode. The plate face of the flexible flange plate is connected with the lower surface of the multi-hole operation platform, the multi-hole operation platform is connected with the flexible film channel through the flexible flange plate, and the multi-hole operation platform rotates with the flexible flange plate as a center axis. The novel multichannel one-hole endoscope puncture outfit is simple in structure and convenient to operate, and when a doctor needs to change an angle to carry out operation in the actual operation process, operation angle regulation can be completed only by rotating the multi-hole operation platform.