The invention discloses an analyzing method for interaction of a DNA probe and an electrode surface based on electrogenerated chemiluminescence of a porphyrin and nucleic acid compound nanometer structure. According to the analyzing method, through the sequence design, the characteristic of DNA base complementary pairing is utilized to self assemble to form a specific nanometer structure; on the basis of the nanometer structure, zinc porphyrin is equimolar assembled with the DNA probe in a G-four-chain manner; zinc porphyrin having the characteristic of generating electrogenerated chemiluminescence is taken as a signal source; the signal strengths of different DNA probes can be detected; the design for the DNA probe is further optimized by analyzing the signal strength. According to the analyzing method, the ECL detection manner is adopted for directly representing the relation between the DNA probe interface and the signal strength, the method is more approaching to the practical detection system and the method can be applied for designing and verifying the DNA probe.