The invention discloses a biological indicator detection device for monitoring the sterilization effect of a lumen instrument. The biological indicator detection device comprises a micropore long-distance spiral lumen cover, a connector and a biological indication cavity, wherein a spiral lumen channel is formed in the micropore long-distance spiral lumen cover; a first air inlet hole, a second air inlet and an air outlet hole are formed in the micropore long-distance spiral lumen cover; the air outlet hole is connected with the connector; a soft sealing rubber plug, a degerming filtration film, a culture medium bag and a biological indicator carrier are arranged in the biological indication cavity. The biological indicator detection device is used for simulating the lumen instrument; the actual working length of the spiral lumen channel can be adjusted, so that the structure of the lumen channel is closer to that of the lumen instrument to be sterilized, and the reliability of a sterilization detection result can be improved; furthermore, the biological indicator detection device can effectively prevent microorganisms such as external germ from entering the biological indication cavity and interfering the detection result. The biological indicator detection device is small in size, convenient to use and easy to operate, and has extremely high popularization and application value.