The invention relates to a pollen-free lily variety breeding method and belongs to the technical field of breeding of new plant varieties. Lily is a main commercial flower in the world, is widely planted around China and occupies huge market shares. However, due to the fact that lily pollen easily stains clothes, the clothes stained with pollen is inconvenient to clean, the lily sold in the market generally undergoes artificial castration processing, the process is complicated and a large number of manpower and material resources is wasted, a bred pollen-free lily is always the key direction of lily breeding, and meanwhile the pollen-free lily having excellent ornamental characteristics also has a huge market prospect. The method creatively utilizes special lily crossbreeding combination, combines with in-bottle sowing, lily tissue culture and breeding, cold storage and vernalization of small lily bulblets, lily seed ball cultivation, pollen-free lily screening and propagation expanding technologies, a breeding period of the pollen-free lily is creatively shortened, and the new pollen-free lily variety having excellent ornamental characteristics is bred.