The invention relates to a Lilium sargentiae Wilson anti-Lily-Fusarium-Wilt related gene KTI EST-PCR (expressed sequence tag-polymerase chain reaction) molecular marker and application thereof, belonging to the technical field of gene engineering. The nucleotide sequence of the KTI gene is disclosed as SEQ ID NO.1. The molecular marker primers are selected from the following primer pair: forward primer: 5'-agatgtctgtggtgctg-3'; and reverse primer: 5'-gatttccgttgagtgtc-3'. The molecular marker primers are utilized to perform PCR amplification on different lily resources, and can be used for identifying different lily-fusarium-disease-resistant/infected lily resources on the molecular level according to the PCR amplification product strip. The molecular marker can also be used for identifying and distinguishing different lily strains, such as Lilium Oriental hybrids, Lilium Asiatic hybrids, LA strains and part of species of wild lilies. The molecular marker primers can provide certain help for selecting the parent materials in the disease-resistance breeding and lily crossbreeding processes.