The invention relates to an animal acoustic system simulation device, which comprises a biological larynx sound production simulator, an animal external ear sound feature simulator and a control device, wherein the biological larynx sound production simulator comprises a simulated larynx for generating simulated sound waves; the animal external ear sound feature simulator comprises an external ear movement device, the external ear movement device comprises a first traction mechanism, a second traction mechanism and a simulated external ear, and a sound wave detector is arranged at the simulated external ear root part for measuring the frequency of the sound waves received by the simulated external ear; the control device comprises a first traction controller, a second traction controller, a first six-degree-of-freedom controller and a second six-degree-of-freedom controller; each traction controller controls the first traction mechanism or the second traction mechanism to drive the simulated external ear to move; and the first six-degree-of-freedom controller and the second six-degree-of-freedom controller are used for controlling two six-degree-of-freedom platforms to change the spatial position of arytenoid cartilage.