The invention provides a method for pilot test amplification scale clarification and filtration of an anti PD-1 monoclonal antibody, wherein the method adopts D0HC and A1HC filter film two-level deep filtration, through small test simulation of the process of deep filtration, a data fitting mathematical equation obtained from testing is used for calculating to obtain the area of a deep filter film required for pilot test scale; at the same time, other process parameters are optimized to obtain the method; the method can use the deep filter film with the area as small as possible during pilot test amplification scale clarification and filtration of the anti PD-1 monoclonal antibody, and saves the cost of production; the end point of the filter film pressure is not greater than 15 psi, the turbidity of a filtration and clarification liquid is less than 20 NTU, and the method is suitable for industrialized amplified production.