The embodiment of the invention discloses a vehicle and vehicle controller interface debugging device. The device comprises a first joint, a second joint, a serial port and a switching circuit. The first joint is connected with the speed measurement test interface of a vehicle controller (VOBC), the second joint is connected with other interfaces of the VOBC except the speed measurement test interface, and the serial port is connected with a test computer and is configured to test the test signals sent by the test computer; and the switching circuit is configured to process the target interface of the VOBC corresponding to the test signals according to the test signals received from the serial port, determining the target joint corresponding to the target interface, and sending the test signals to the target interface through the target interface. The vehicle and vehicle controller interface debugging device is connected with the VOBC through the first joint and the second joint and employs the switching circuit to complete the switching so as to reduce the number of the external interfaces of the debugging device, avoid large economic losses caused by burning of VOBC when a vehicle circuit has problems; and moreover, the program debugging preparation of the debugging device and the VOBC is finished prior to the test to greatly reduce the debugging preparation time of the test site.