The invention belongs to the field of biological medicine, and in particular relates to a ursolic acid-containing antibacterial composition and application thereof. The ursolic acid-containing antibacterial composition comprises ursolic acid and an antibiotic, wherein the antibiotic is selected from one of roxithromycin, levofloxacin, amoxicillin, cephradine and gentamicin. With the combination of the ursolic acid and the antibiotic, the sensitivity of staphylococcus aureus to antibiotics can be improved, meanwhile the use amount of the antibiotic can be effectively reduced, adverse drug reactions can be reduced, and the ursolic acid and the antibiotic have a synergistic effect, so that the ursolic acid-containing antibacterial composition has good application prospects and is applicable to large-scale popularization and application.