利索-全球专利检索

  1. 登录
  2. 注册
发明授权
CN106701810B 一种谷氨酸棒状杆菌的基因编辑系统及其应用 有权

一种谷氨酸棒状杆菌的基因编辑系统及其应用
摘要：
本发明提供了一种谷氨酸棒状杆菌的基因编辑系统，其具有较高的基因编辑效率。谷氨酸棒状杆菌中待编辑的目的基因具有5′‑（N20）‑NGG‑3′结构，N为A、T、C或G，该基因编辑系统包括cas9表达载体和sgRNA表达载体；cas9表达载体包括cas9序列和SD序列，cas9序列如SEQ ID No.1所示，SD序列如SEQ ID No.2所示、并连接在cas9序列的始密码子ATG前；sgRNA表达载体的包含sgRNA序列和同源修复序列，sgRNA序列为crRNA与trancrRNA 连接形成的复合体序列，trancrRNA序列如SEQ ID No.3所示，crRNA序列为20bp的引导序列、与目的基因的N20序列相同。
摘要（英）：

The invention provides a gene editing system of Corynebacterium.Glutamicum, which is relatively high in gene-editing efficiency. A to-be-edited target gene in the Corynebacterium. Glutamicum has a 5'-(N20)-NGG-3'structure, N is A, T, C or G, and the gene editing system comprises a cas9 expression vector and an sgRNA expression vector; the cas9 expression vector comprises a cas9 sequence and an SD sequence, the cas9 sequence is as shown in SEQ ID No. 1, and the SD sequence is as shown in SEQ ID No. 2, and linked before a start codon ATG of the cas9 sequence; the sgRNA expression vector comprises an sgRNA sequence and a homologous repair sequence, the sgRNA sequence is a complex sequence formed by linkage of crRNA and trancrRNA, the trancrRNA sequence is as shown in SEQ ID No. 3, and the crRNA sequence is a guide sequence of 20 bp and the same as an N20 sequence of the target gene.

公开/授权文献
0/0